The increased concentration of carbohydrate and proteose peptone helps to reduce the inhibitory effect of bile salts and indicators and allows good growth of salmonella and shigella species while inhibiting the normal intestinal flora. Fire fighting measures personal precautions wear disposable gloves, dust mask to en149 ffp2s and eye protection. Hektoen enteric agar is not intended for use in the diagnosis of disease or other conditions in humans. Hektoen enteric agar was formulated by kind and metzger and meets the requirements of the american public health association apha. Summary and explanation hektoen enteric he agar was developed by king and metzger to produce a medium simultaneously conducive to. Hektoen enteric agar was developed in 1967 by king and metzger. Summary and explanation through the years many media have been devised. Hektoen enteric agar for the detection and isolation of pathogenic intestinal bacteria find msds or sds, a coa, data sheets and more information.
Hektoen enteric agar is a selective medium for isolation and differentiation of gramnegative enteric. The incorporation of larger quantities of peptone to counteract the. For the isolation and cultivation of gramnegative enteric microorganisms, especially shigella, from a variety of clinical and nonclinical specimens. Bile salts and the dyes bromthymol blue and acid fuchsin inihibit the growth of most gram positive organisms.
Brilliant green bg agar and hektoen enteric he agar media were compared for their efficiency in isolating salmonellae from various food products. Hektoen enteric agar is a selective and differential medium designed to isolate and differentiate members of the species salmonella and shigella from other enterobacteriaceae. Hektoen enteric agar for selective, differential isolation of salmonella and shigella. Hektoen enteric he agar intended use remel hektoen enteric he agar is a solid medium recommended for use in qualitative procedures for the isolation and differentiation of gramnegative enteric pathogens. Hektoen enteric agar hek, he or hea is a selective and differential agar primarily used to recover salmonella and shigella from patient specimens. Hektoen enteric agar for microbiology sigmaaldrich. Hektoen enteric he agar is a medium for the isolation of shigella and salmonella. Hektoen enteric agar culture media online microbiology. Fight larger fires with water spray or alcohol resistant foam.
Hektoen enteric agar is a type of primary standard media, which may be commonly used in the selective detection and isolation of salmonella in food microbiology labs and in human stool specimens. Of the 11,226 food specimens examined, 1,662 or 14. Learn vocabulary, terms, and more with flashcards, games, and other study tools. Hektoen enteric agar is a moderately selective medium used for the isolation and cultivation of gramnegative enteric microorganisms, especially shigella spp, from faeces, foodstuffs and other materials of sanitary importance. Hektoen enteric agar, agar hektoen enterico, product. Hektoen enteric agar is a differential and selective medium for the isolation of shigella and salmonella spp easytoread. Other companies are charging thousands of dollars to set up accounts and give you access to their msds online database.
Product instructions bp0230500 hektoen enteric agar gelose. Hektoen enteric agar microbiology food and drug reference. Comparison of brilliant green agar and hektoen enteric. Hardy diagnostics hektoen enteric he agar is a selective and differential medium used for the isolation and differentiation of gramnegative enteric pathogens. Hektoen enteric agar print share bd bbl prepared plated media. The ph indicator system consists of acid fuchsin and bromthymol blue. All stock cultures 100%, including cultures of h 2 snegative isolates, yielded.
Hea contains indicators of lactose fermentation and hydrogen sulfide production. Bd hektoen enteric agar he agar intended use bd hektoen enteric agar is a moderately selective and differential medium for the isolation and cultivation of gramnegative enteric microorganisms, especially for the isolation of shigella and salmonella species from fecal specimens. Your new online msds binder is a place for you to store the material safety data sheets you need to deploy. Hektoen enteric agar safety data sheet date of issue 17022009. When compared with other selective medium, this medium inhibits the growth of salmonella and shigella very slightly.
Evaluation of a novel chromogenic medium as a replacement. Hektoen enteric agar references the powder is very hygroscopic. Product summary and explanation hektoen enteric agar was developed in 1967 by king and metzger. It relies on the use of bile salts for selective inhibition and two indicator systems. Hektoen enteric he agar is a medium that relies on the use of.
This chapter discusses that hektoee enteric he agar relies on the use of bile salts for selective inhibition and two indicator systems. The selective and differential aspects of this complex medium are due to presence of the dyes acid fuchsin and bromthymol blue, the sugars lactose, sucrose and salicin, and the compounds sodium thiosulfate and ferric ammonium citrate and bile salts. Chromagar salmonella cas, a new chromogenic medium, was retrospectively compared to hektoen enteric agar hea with 501 salmonella stock isolates and was then prospectively compared to hea for the detection and presumptive identification of salmonella spp. The additional carbohydrates sucrose and salicin give better differentiation than lactose alone and the lower toxicity of the double indicator improves recovery. Hektoen enteric agar as part of the multistep procedure to isolate salmonella 1,10,12. Hektoen enteric agar productinformation product number h7532 product description hektoen enteric agar is a differential selective medium used for the isolation of shigella and salmonella species from enteric pathological specimens. En caso negativo, incubar nuevamente durante 18 a 24 horas mas. Hektoen enteric he agar for stool cultures for the. The medium was introduced in 1968 by sylvia king and william i. Hektoen enteric agar, a selective and differential medium designed to isolate and differentiate members of the species salmonella and shigella from other enterobacteriaceae and was developed by king and metzger 1,2. Hektoen enteric agar intended use hektoen enteric agar is a selective medium for the isolation and differentiation of pathogenic enterobacteria from biological samples of animal origin, water samples, dairy products and other food products. Hektoen enteric agar is used for the isolation and differentiation of enteric pathogens.
Hektoen enteric agar was formulated in 1967 by king and metzger at the hektoen institute in order to increase the isolation frequency of shigella and salmonella species in comparison to that obtained with other selective isolation media. King and metzger developed he agar in an effort to increase the recovery of salmonella and shigella species over the previously formulated salmonellashigella ss agar. It is named after the hektoen institute in chicago, where researchers developed the agar. Bd bbl hektoen enteric agar is a moderately selective and differential medium for the isolation, cultivation.
Learn hektoen enteric agar with free interactive flashcards. See section 8 for information on personal protection equipment. Hektoen enteric agar is a differential selective medium used for the isolation of shigella and salmonella species from enteric pathological specimens. Animal peptones and yeast extract provide the nutritive base. Hektoen enteric agar was developed in 1967 by king and metzger of the hektoen institute in order to increase the frequencies of isolation of shigella and salmonella organisms when compared with their recovery on other media frequently utilized in clinical laboratories at that time 57. Hektoen enteric agar was originally developed by king and metzger 1, 2.
Cm0419 extinguishing media suitable use dry chemicals, co2, water spray or foam. The presence of the bile salts and dyes inhibit most grampositive organisms allowing only gramnegative rods to grow on he agar. Summary and explanation hektoen enteric he agar was developed by king and metzger to produce a medium simultaneously conducive to growth of salmonella and. Compared to other enteric differentiating media commonly used, hektoen enteric agar. The high peptone content offsets the inhibitory effect of bile salts on shigella species in particular. Hektoen enteric agar is a selective as well as differential media for the isolation and differentiation of enteric pathogens from clinical specimens. Comparison of chromagar salmonella medium and hektoen. Although this medium enabled a wide variety of pathogenic. Hektoen enteric agar 78 intended use hektoen enteric agar is used for the isolation and differentiation of enteric pathogens. Product instructions en hektoen enteric agar fr gelose. Hektoen enteric agar is a selective and differential media for the recovery of enteric gramnegative rods from mixed microbiota. Hektoen enteric agar he is a differential and selective plating medium for culture of enteric pathogens. Other notes plating medium for the isolation of enteric pathogens.
Hektoen enteric agar 3 product instructions description medium used in qualitative procedures for the isolation of gram negative enteric microorganisms, especially salmonella and shigella species. Shop online for a wide selection of bd difco dehydrated culture media. Remel hektoen enteric he agar is a solid medium recommended for use in qualitative procedures for the isolation and differentiation of gramnegative enteric pathogens. The growth of grampositive organisms and nonpathogenic enteric coliforms is inhibited through the use of bile salts and dyes, allowing intestinal pathogens, such as salmonella and shigella, to be more easily recovered. The medium is particularly suited for the culture of shigella, and prevents invasion by proteus.
Preparation of the medium suspend 75 grams of the powder in 1. Hektoen enteric agar he is a selective and differential medium designed to isolate and differentiate members of the species salmonella and shigella from other enterobacteriaceae. Hektoen enteric agar is a selective as well as differential media for the isolation and differentiation of enteric pathogens from clinical. Hektoen enteric agar was formulated by kind and metzger and meets the requirements of the. Hardychrom ss is a reliable and economical method for the selective isolation and differentiation of salmonella and shigella spp.
An effort to develop a medium which would be conducive to growth of shigellae and inhibitory to normal intestinal flora was reported by king and metzger. Hektoen enteric agar is a selective and differential media for the isolation and differentiation of enteric pathogens from clinical specimens. Pi 78, rev 01, 120203 hektoen enteric agar 78 intended use hektoen enteric agar is used for the isolation and differentiation of enteric pathogens. The additional carbohydrates sucrose and salicin give better differentiation than lactose alone and the lower. Comparison of chromagar salmonella medium and hektoen enteric. Choose from 159 different sets of hektoen enteric agar flashcards on quizlet. It relies on the use of bile salts for selective inhibition and two indicator. Hektoen enteric hicynth agar is the modification of the. Principles and explanation of the procedure microbiological method.
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